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Home page > Equipment and Reservation > HPLC

HPLC

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1 Analytical RP-HPLC (Alliance, Waters)
- degas and mix of up to 4 eluents -50 µL to 5 mL/min
- up to 120 samples -automatic injection from 1 to several hundreds of µL -diode array detector (UV-Vis)

Semi-preparative RP-HPLC systems (Waters)
- automated fraction collector by time-from 6 to 50 mL/min -columns : C18, C4, C3 + 1 column C18 radial compression
- purification of 80 mg to 1 g raw material
- purification with argon-degassed eluents
- UV/Vis detector (190 to 700 nm) Semi-prep1 Semi-prep2

Automated preparative LC-MS system (Waters)
- automated fraction collector by mass-25 mL/min -columns : C18
- purification of 80 mg to 1 g raw material
- UV/Vis detector (190 to 700 nm)

1 nano HPLC system (LC Packings)
- for proteomic research : polypeptides separation (ng) before MS analysis -UV detector
- 3 modes :
- Mode 1 : 1 mm (diam) columns, flow 40 µL/min, 5-100 pmoles peptide sensibility
- Mode 2: 250 microns (diam) columns, flow 2-3 µL/min, 1 pmole peptide sensibility
- Mode 3: 100 microns (diam) columns, flow 0.2 µL/min, <1 pmole peptide sensibility NanoHPLC